Populating a Project: ISA Structure

Populating a Project: ISA Structure#

Purpose: To populate a project with the experimental information to organise your data and related information. Once your project has been created you are now ready to populate your project and create the ISA (Investigation,Study, Assay) structure

There are multiple ways of doing this:

  1. You can either create this structure manually

    OR

  2. Use the FAIR Data Sation

Populating the project Manually#

This is where we will create the ISA structure. The ISA (Investigation, Study, Assay) is a general purpose framework for describing how experiments relate to one another.

Step 1: Create and Investigation#

Click on the “Create” tab and from the pull-down menu select “Investigation” and a New Investigation page will open. Fill in the following sections with relevant information:

Title Investigation Name: Understanding the extracellular environment in Aspergillus niger

Description: An experimental validation of the predicted proteome confirmed in silico predictions.

Extended metadata: If applicable

Investigation position: You can specify the position you want this Investigation to have on the tree : 1

Project: Assign the Investigation to the project created

Sharing: Add the sharing permissions you want to associate with the project

Sharing permissions

Creators: Add other creators e.g. co-investigators o

Creators overview

Publications: Add any relevant publications that will come out of this project or the ones this project is based on.

Hit the Create button and your Investigation will be created.

Created investigation

Step 2: Create the Study#

A study is a particular biological hypothesis or analysis. Multiple studies can be connected to an investigation. After the Investigation has been created you will now create a Study and associate it with the Investigation created above.

Click on the “Create” tab and from the pull-down menu select “Study” OR from the Investigation created above click on the Add new button and a New Study page will appear.

Fill in the relevant sections like in the Investigation above:

Title: In silico prediction

Description:

Extended metadata: If applicable

Study position: You can specify the position you want this Study to have under the Investigation: 1

Project: Assign the Investigation to the project created

Sharing: Assign the who you want to share the data with.

Creators: *Add additional creators if you wish. *

Publications:

  • Add any associated Publications.*

Step 3: Create the Assays#

Multiple assays can be connected to a Study. Experimental assays refer specifically to laboratory assays. Click on the “Create” tab and from the pull-down menu select “Assay” OR from the Study created above click on the Add new button and select Add new Assay

Fill in the relevant sections like in the Investigation above:

Title: Signal Peptide Predictions

Description:

Extended metadata: If applicable

Study position: You can specify the position you want this Study to have under the Investigation: 1

Project: Assign the Investigation to the project created

Sharing:

Creators:

Publications:

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